Regulatory

Part:BBa_K4073001:Design

Designed by: Ananya Bharathwaj   Group: iGEM21_WVHS_SanDiego_CA   (2021-09-26)


Promoter expresses genes when the salt threshold is reached.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 755
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 225


Design Notes

The main design considerations we had to deal with include where the restriction enzymes for the CrGPDH3 promoter would be. For example, both RIA1 and RIA3 were regions of interest, and so we had to decide which one of them would yield the most enhanced salinity mechanisms in the form of NaCl response elements. We ended up choosing the restriction enzymes SpeI to KpnI, encompassing both the RIA1 and the RIA3 regions, for the maximum effect of the mechanisms under theoretical NaCl treatments.


Source

The promoter was derived from the NCBI sequence (​​https://www.ncbi.nlm.nih.gov/nuccore/1601834299) for the CrGPDH3 salt-inducible promoter. More information about the characteristics of the CrGPDH3 promoter (RIA1 & RIA3 regions) can be found on this paper (https://link.springer.com/content/pdf/10.1007/s00253-019-09733-y.pdf).

References